Item – Theses Canada

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OCLC number
28499210
Author
Boone, David,1965-
Title
Regulation of steroidogenesis in the corpus luteum of the pseudopregnant rat.
Degree
M. Sc. -- University of Waterloo, 1991
Publisher
Ottawa : National Library of Canada = Bibliothèque nationale du Canada, 1992.
Description
2 microfiches.
Notes
University Microfilms order no. UMI00307568.
Includes bibliographical references.
Abstract
The regulation of steroidogenesis in rat luteal tissue of different ages was studied. Prostaglandin F2 alpha (PGF2a) inhibited LH stimulation of progesterone production only in 7 day old luteal dispersions. Pertussis toxin augmented the ability of PGF2a to be luteolytic in vitro. Arachidonic acid inhibited LH stimulation of progesterone production in 3 day but not 5 day old tissue. The lipophilic antioxidant lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), also stimulated progesterone production. NDGA stimulation did not change significantly from 3 to 8 days of luteal age and NDGA did not stimulate cAMP production. PGF2a also inhibited NDGA stimulation in 7 day old luteal tissue only. Other inhibitors of lipoxygenase (esculetin, baicalein, BW755C), cyclooxygenase (indomethacin, BW755C) or phospholipase A$\sb2$ (bromophenacyl bromide) did not mimic the stimulatory effect of NDGA. The lipoxygenase products, leukotrienes A$\sb4$ to E$\sb4$, did not counter the stimulation by NDGA. Calmidazolium also stimulated progesterone production. In conclusion, the functional character of luteal tissue varied with age from 3 to 8 days. Age dependent changes, such as those with LH stimulation, were not found with NDGA stimulation and NDGA stimulation did not involve cAMP accumulation. (Abstract shortened by UMI.)
ISBN
0315691190
9780315691193