Item – Theses Canada

OCLC number
1032963447
Link(s) to full text
LAC copy
LAC copy
Author
Staudinger, Lisa Alexandra.
Title
Functional Interactions between the Discoidin Domain Receptor 1 and Beta 1 Integrins.
Degree
MAST -- University of Toronto, 2013
Publisher
Toronto : University of Toronto, 2013.
Description
1 online resource
Notes
Includes bibliographical references.
Abstract
The rate limiting step of phagocytosis is the binding of collagen to specific receptors, which include [beta]1 integrins and the discoidin domain receptor 1 (DDR1). While these two receptors may interact, the functional nature of these interactions is not defined. We examined the effects of DDR1 over-expression on [beta]1 integrin function and determined that DDR1 over-expression enhanced cell attachment through [beta]1 integrins. These data are consistent with data showing that DDR1 over-expression enhanced cell-surface, but not total, [beta]1 integrin expression and activation. As shown by experiments with endoglycosidase H, DDR1 over-expression increased glycosylation of the [beta]1 integrin subunit. Collectively these data indicate that DDR1 enhances [beta]1 integrin interactions with fibrillar collagen, possibly by affecting the processing and trafficking of [beta]1 integrins to the cell surface. Our data provide insight into the mechanisms by which fibrotic conditions such as cyclosporine A-induced gingival overgrowth are regulated.
Other link(s)
hdl.handle.net
tspace.library.utoronto.ca
Subject
Collagen.
Discoidin Domain Receptor 1.
Beta 1 Integrins.
Remodeling.
Glycosylation.
Phagocytosis.
0307.
0379.